BCMB 271 -Biochemistry 1
A comprehensive survey of biochemistry and molecular biology including protein structure, enzyme kinetics, membrane structure, DNA structure, replication, transcription and translation. The laboratory introduces basic techniques including buffer preparation, protein isolation and identification, purification of enzymes, enzyme kinetics, gel electrophoresis, column chromatography and DNA isolation and characterization.
Research Methods Instrumentation: Performing bioluminescence assays to measure kinase activity and effect of small molecule kinase inhibitor using Spectramax M5
In this course, you will get acquainted with a gold standard instrument used in a majority of industrial and academic laboratories, the Spectramax M5 plate reader. This instrument can do measurements of luminescence, time-resolved fluorescence, fluorescence polarization, and Homogeneous Time Resolved Fluorescence, in addition to measuring standard absorbance. You will learn to operate the instrument and learn to use the software of the system. You will learn to use measure kinase activity using bioluminescence as a readout on the SpectraMax M5, and do an assay to measure the effect of an inhibitor on the kinase activity. This instrumentation course is recommended for graduate students, and for seniors and juniors who plan to work in an industrial biotech laboratory setting or pursue higher studies.
BCMB 276/376: Chemical Biology – techniques and applications in research
An in-depth discussion of tools and techniques of chemical biology and its applications in research, with a focus on cancer research. After discussion of the chemical tools, we shall study current research utilizing these tools to address biological problems. Some techniques that will be covered are chemical and biological library synthesis, library screening methods, quantitative techniques such as Fluorescence polarization, Surface Plasmon Resonance, Isothermal Calorimetry. Biological research problems such as kinase inhibition, modulation of protein protein interactions, targeted protein degradation in live cells and live cell imaging using bio-orthogonal reporters will be discussed.
Research Methods: Instrumentation: High Performance Liquid Chromatography
In this course, we shall learn the principles behind High Performance Liquid Chromatography (HPLC), learn to operate a High Performance Liquid Chromatography system, do sample separation using High Performance Liquid Chromatography, and analyze the results.
By the end of the course, the student will:
1) Have an understanding of the theory behind High Performance Liquid Chromatography.
2) Be able to operate Agilent HPLC system and use Agilent HPLC software
3) Be able to develop a gradient for separation of peptide samples and make a customized method in the HPLC software.
4) Be able to run peptide sample on the HPLC and analyze the result of the run.
5) Be able to compare their run to other provided HPLC runs and be able to interpret the nature of their sample.
BCMB 271: Biochemistry 1